Overview

Mixing cells with the bioink is a critical step for 3D bioprinting. Because of the differences in viscosity of the cell suspension and bioink, the traditional manual mixing methods create a lot of air bubbles and non-uniform mixture which leads to the unstable printed structure, difficulty for cell observation and affect cell viability.

The VitroINK Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK and cell suspension are placed in the cell mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

The syringe, connector and mixing head are disposable for one-time use. Order the sterilized replacement mixing kit components to ensure the robust results for every single mixing.

Kit Contents & Specifications

• Disposable mixing kit components for VitroINK bioink:
  (1) Cell mixer dispenser
  (1) 1 mL syringe
  (1) 3 mL syringe
  (1) connector and tubing
  (1) mixing head

• Sterile

• Use for 3:1 or 10:1 mixing ratio

• Ships room temperature. Store at room temperature.

DATA AND REFERENCES

VitroINK Mixing Kit Demonstration