Plate Size: 6-well

SKU: VP-SA6W

Plate Size: 12-well

SKU: VP-SA12W

Plate Size: 24-well

SKU: VP-SA24W

Plate Size: 48-well

SKU: VP-SA48W

Plate Size: 96-well

SKU: VP-SA96W

VitroPrime™ Spread-Attach Cell Culture Plates

The VitroPrime™ Spread-Attach plates offer unparalleled advantages for hydrogel-based 3D cell culture. Experience swift and homogeneous hydrogel spreading, ensuring impeccable attachment and mitigating the notorious gel floating issue. The plate is engineered to provide a seamless and uniform environment for your hydrogel-based experiments, thus eliminating the edge effects for flat gel surfaces.

Beyond its specialty in 3D cell culture, VitroPrime™ Spread-Attach plates excel in 2D cell culture applications, setting a new standard for cell adherence. The unique surface treatment of these plates ensures superior cell adherence, promoting rapid growth and heightened yields, especially for cell types that are adherent cultures involving primary cells, neuronal cells, stem cells, and other challenging cell types. The versatility of VitroPrime™ Spread-Attach Plates extends to adapting quickly to serum-free or low-serum culture conditions, making them indispensable for experiments seeking to minimize serum interference.

Particularly optimized for xeno-free biofunctional VitroGel systems, the combination of VitroGel and the VitroPrime™ Spread-Attach plates can unlock the full automation potential of hydrogel-based 3D cell culture.

RESOURCES

DATA AND REFERENCES

Figure 1. A comparison of two 25-stitched z-planes within a well of a 12-well plate
A. Standard plate #1 shows uneven hydrogel surface by showing attach cells in different focus plane. B. VitroPrime™ Spread-Attach 12-Well plate. shows flat hydrogel surface by presenting homogeous cell spreading and attachment on the same plane.

Evaluating Gel Spreading and Cell Attachment - VutroPrime Spread-Attach 12-Well v. Standard Plate

We meticulously compared two 25-stitched z-planes capturing OP9 cells in the VitroGel Hydrogel Matrix within a 12-well plate—Standard Plate #1 and VitroPrime™ Spread-Attach Plate 12-Well. The images were compiled by selecting the z-plane with the 2D projection on each plate and stitched to evaluate cell attachment and the flatness of the hydrogel surface. Notably, VitroPrime™ Spread-Attach 12-Well plate demonstrated superior performance, ensuring flat hydrogel surface for even gel spreading and cell attachment.

Figure 2. Comparison of hydrogel adherence on four different quality 24-well plates in 48 hours.
A. Standard Plate #1 demonstrates hydrogel adherence in 21 out of 24 wells. B. Standard Plate #2 exhibits hydrogel adherence in 22 out of 24 wells.
C. Premium Plate #1 showcases hydrogel adherence in 23 out of 24 wells. D. VitroPrime™ Spread-Attach 24-Well plate exhibit flawless hydrogel adherence in all 24 wells.

Comparison of Hydrogel Adherance – VitroPrimeTM Spread-Attach v. Others

We investigated the effectiveness of VitroPrime Spread-Attach and three other plasticswares in mitigating the hydrogel floating issue using VitroGel ORGANOID-3 (VHM04-3) hydrogel.

The method involves combining hydrogel and basal medium in a 2:1 ratio, followed by adding 300 uL of VitroGel ORGANOID-3 hydrogel mixture to each well of a 24-well plate. The hydrogel is incubated for 20 minutes, after which 300 uL of cover medium is added on top. The plates are then incubated at 37°C to assess the ability of VitroPrime plasticware to prevent hydrogel floating.

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CELL CULTURE INSERTS

VitroPrime™ Cell Culture Inserts

Transparent, PET, Sterile, 12/PK, 48/CS