VitroGel® COL High Concentration

VitroGel COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel COL High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Mix & Match – 3D Cell Culture Your WAY!

Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® COL can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® MMP to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.

Learn more about the “Mix & Match” features >

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength

Cell Type Behavior Reference Table – VitroGel COL

Studies performed using VitroGel COL in different tissue and cell types.

DATA AND REFERENCES

All Cell type Behaviors for All VitroGel Products

DATA

Figure 1. Rheological properties of VitroGel COL with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel COL was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio. B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test.

Figure 2. 3D culture of mouse bone marrow stromal cells (OP9 mesenchyme) in the mixture of VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL The single cells were suspended in hydrogel matrix (Day 1) and extended to form a fibroblast-like cell-matrix structure (Day 7). “

Figure 3. 3D culture of glioblastoma cells (SNB-75) in VitroGel COL.
Cells were cultured with 1:5 diluted VitroGel COL follow the user handbook (50% FBS was used to prepare the cell suspension to get hydrogel with final 10% FBS concentration).

REFERENCES/PUBLICATIONS

• Powell K.(2017) Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96

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