VitroGel® IKVAV High Concentration

VitroGel® YIGSR High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration. VitroGel YIGSR High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa. 

VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Mix & Match – 3D Cell Culture Your WAY!

Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® YIGSR can be “mix & matched” with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can tailor create their 3D culture micro-environment for different applications.

Learn more about the “Mix & Match” features >

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength

DATA AND REFERENCES

Cell Type Behavior Reference Table – VitroGel YIGSR

Multiple uses of immobilized conjugated YIGSR in different tissue and cell type. YIGSR has been used as an immobilized, adhesive substrata for multiple cell types to study many different cellular processes and behaviors in normal physiological and pathological contexts.

All Cell type Behaviors for All VitroGel Products

DATA

Figure 1. Rheological properties of VitroGel YIGSR with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel YIGSR was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)

Figure 2. 3D culture of glioblastoma cells (U-87 MG) in VitroGel YIGSR.
Cells were cultured with 1:3 diluted VitroGel YIGSR according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).

Figure 3. 3D culture of breast cancer cells (MCF-7) in VitroGel YIGSR.
Cells were cultured with 1:3 diluted VitroGel YIGSR according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).

REFERENCES/PUBLICATIONS

• Powell K.(2017) Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96

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