VitroGel® RGD High Concentration

RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)

High Concentration Kit Type: VitroGel RGD + Dilution Solution TYPE 1/TYPE 2

CATEGORY: High Concentration Hydrogels

VitroGel RGD + Dilution Solution TYPE 1 SKU: TWG003

Dilution Solution TYPE 1 contains sucrose for best osmolarity

VitroGel RGD + Dilution Solution TYPE 2 SKU: TWG003-2

Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® RGD High Concentration

VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa. 

VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Mix & Match – 3D Cell Culture Your WAY!

Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® RGD High Concentration can be “mix & matched” with other VitroGel High Concentration hydrogels such as VitroGel® IKVAV, VitroGel® YIGSR, VitroGel® MMP, and VitroGel® COL to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists customize their 3D culture micro-environment for different applications.

Learn more about the “Mix & Match” features >

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength

Cell Type Behavior Reference Table – VitroGel RGD

Multiple studies have made use of RGD hydrogel in different tissue and cell types. RGD is commonly used as an immobilized, adhesive ligand in 3D hydrogels that allows researcher to study many different cellular processes and behaviors in normal physiological and pathological contexts.

DATA AND REFERENCES

Figure 1. Rheological properties of VitroGel RGD with DMEM medium.
A) – C) The gel formation curve after mixing with DMEM (A), DMEM/F-12 (B), and RPMI (C) media. VitroGel RGD was diluted at 1:0,1:1, 1:2 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with media at 4:1 (v/v) ratio; D) – F) The gel strength after 24 hrs incubation in DMEM (D), DMEM/F-12 (E), and RPMI (F) media. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)

DATA

Figure 2. 3D culture of OP9 cells in VitroGel RGD.
Hydrogel was prepared at 1:3 dilution with VitroGel Dilution Solution (Type 1). The images were taken on days 2 and 7. VitroGel RGD shows support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure.
Figure 3. 3D view of OP9 cells growth in VitroGel RGD.
Cell networking structure formed in VitroGel RGD.
Figure 4. 3D culture of U87-MG cells in VitroGel RGD.
Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of VitroGel RGD. U87-MG cells shows cell networking structure and the cell morphology in VitroGel RGD indicating a cell-cell and cell-matrix interaction.

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