Pore Size: 3 µm

3 µm pore size is ideal for transport, transport/co-cultivation, tissue engineering, chemotaxis, haptotaxis, and invasion studies

SKU: VPE3-24-4

Pore Size: 8 µm

8 µm pore size is ideal for tumor cell migration and invasion, co-culture, chemotaxis and haptotaxis studies.

SKU: VPE8-24-4

VitroPrime™ Cell Culture Inserts

VitroPrime™ Cell Culture Inserts closely mimic the in vivo environment, promoting enhanced attachment, growth, and differentiation of a wide range of cell types. Their permeable nature makes inserts a convenient and powerful resource for various research applications, including studies related to cell migration and invasion.

• Excellent-quality images can be taken with VitroPrime™ Cell Culture Inserts.

• – The membrane does not stained when performing common staining for imaging (i.e., crystal violet).
  – Images can be taken without removing the insert from the plate.

• Even pore sizes throughout the membrane provide accurate results when evaluating cell invasion.

• Medium can be added to the outer wells without removing the inserts.

• The inserts support and sustain proper solidification of the hydrogel matrices.

Cell Culture Inserts Applications:

• Co-Culture Studies: Investigate complex cell interactions and microenvironment effects with precision.

• Drug Screening: Screen potential pharmaceutical compounds with confidence.

• Migration and Invasion Assays: Study cell mobility and invasiveness in a controlled environment.

• Tissue Engineering: Create tissue models and explore regenerative medicine applications.

DATA AND REFERENCES

Figure 1. Tube formation of endothelial cells on top of VitroGel AAK-HC hydrogel with tube formation supplement, AAK Supplement 2.
The time-lapse video above shows the tube formation during 18 hours after Human Umbilical Vein Endothelial Cells (HUVEC) seeding on top of VitroGel AAK-HC hydrogel with AAK Supplement 2. The image above shows the tube morphology of HUVEC cells on top of VitroGel AAK-HC hydrogel. The cells were fixed and stained with DAPI (blue) and ActinGreen (green).

Figure 2. HUVEC cell growth on top of VitroGel AAK-HC hydrogel with cell growth supplement, AAK Supplement 1.
The image above shows HUVEC cells attached and grew on the surface of VitroGel AAK-HC hydrogel with cell growth supplement, AAK Supplement 1. The cells were fixed and stained with DRAQ5 (red) and actin green (green).

Figure 4. 3D tube structure of HUVEC cells cultured with the VitroGel Angiogenesis Assay HC Kit.
The concentration of VitroGel AAK-HC hydrogel was adjusted by diluting with AAK-HC Dilution Solution. HUVEC cells were prepared in hydrogel supplement before mixing with diluted hydrogel solution for 3D culture. The video shows the z-stack images of 3D tube structure formed inside of the hydrogel (after 7 days of incubation). The image above shows the tube networking structure inside of VitroGel AAK-HC hydrogel.

RELATED PRODUCTS

ASSAY KITS

VitroGel-Based Cell Invasion Assay Kits

Xeno-free hydrogel system for consistent study of cell invasion. Kit includes choice of hydrogel with VitroPrime™ Cell Culture Inserts, 8 µm, PET, 12/PK.

CELL CULTURE INSERTS

VitroPrime™ Spread-Attach Plate

Xeno-free hydrogel system for consistent study of cell invasion. Kit includes choice of hydrogel with VitroPrime™ Cell Culture Inserts, 8 µm, PET, 12/PK.